Difference between revisions of "Circular chromatography"
From (art)scienceblr
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| + | ==Introduction== | ||
| + | ==Methods=== | ||
This is the recommended protocol from the page of [http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA] | This is the recommended protocol from the page of [http://biodynamics.in/chrom.htm BIO-DYNAMIC ASSOCIATION OF INDIA] | ||
| + | ===Materials=== | ||
| + | ===Step by Step=== | ||
# A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. | # A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. | ||
# The wick is removed and the paper is dried. | # The wick is removed and the paper is dried. | ||
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# When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop. | # When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop. | ||
| − | [[Category:Protocols]] | + | |
| + | [[Category::Protocols]] | ||
Revision as of 10:37, 27 March 2017
Introduction
Methods=
This is the recommended protocol from the page of BIO-DYNAMIC ASSOCIATION OF INDIA
Materials
Step by Step
- A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter.
- The wick is removed and the paper is dried.
- Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time.
- The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper.
- When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop.
[[Category::Protocols]]
