Difference between revisions of "Circular chromatography"
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DAY1<br> | DAY1<br> | ||
====Extraction==== | ====Extraction==== | ||
| − | Just like taking a crushed piece of garlic, and putting it into water or oil, brings out different flavors in the water and oil, we decided to see what | + | <p> |
| + | Just like taking a crushed piece of garlic, and putting it into water or oil, brings out different flavors in the water and oil, we decided to see what will be extracted from the soil samples when using 2 different solutions. | ||
| + | </p> | ||
| + | * 0.2% NaOH in water | ||
| + | * isopropyl alcohol | ||
| + | |||
| + | <p> | ||
| + | We took one spoon scoop of soil/mud into petridish to mix and extract with NaOH.<br> | ||
| + | The same scoop of soil/mud into a tube to extract with isoproypl alcohol (it evaporates faster).<br> | ||
| + | </p> | ||
| + | <p> | ||
| + | Here, it may be best to put both in a closeable container, so we can mix and shake the samples.<br> | ||
| + | We left the samples overnight at room temperature.<br> | ||
| + | </p> | ||
| + | <br> | ||
| + | '''DAY2'''<br> | ||
| − | |||
====Preparation of Light sensitive paper==== | ====Preparation of Light sensitive paper==== | ||
====Sample application==== | ====Sample application==== | ||
Revision as of 11:00, 3 April 2017
Contents
Introduction
CHROMATO = colour; of or in colours
GRAPHY = "writing" or a "field of study"
Methods
This is the recommended protocol from the page of BIO-DYNAMIC ASSOCIATION OF INDIA
Materials
Chemicals
- 0.5% solution of silver nitrate (you can find in photography suppliers)
- 0.1 to 1% solution of sodium hydroxide (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)
Other materials
- paper (Whatman, or any acid-free thick paper)
- reservoir
- wick (medical cotton, and rolled it into a wick)
- light for development - sunlight is fine unless controlled exposure is wanted
Step by Step
DAY1
Extraction
Just like taking a crushed piece of garlic, and putting it into water or oil, brings out different flavors in the water and oil, we decided to see what will be extracted from the soil samples when using 2 different solutions.
- 0.2% NaOH in water
- isopropyl alcohol
We took one spoon scoop of soil/mud into petridish to mix and extract with NaOH.
The same scoop of soil/mud into a tube to extract with isoproypl alcohol (it evaporates faster).
Here, it may be best to put both in a closeable container, so we can mix and shake the samples.
We left the samples overnight at room temperature.
DAY2
Preparation of Light sensitive paper
Sample application
Exposure to see the different bands
The steps from Biodynamics India
- A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter.
- The wick is removed and the paper is dried.
- Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time.
- The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper.
- When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop.
Which method to use
- Bio Crystallisation, copper chloride crystallisation, proteins
- Capillary Dynamolysis, vertical filters, ‘rising pictures’, sugars/ bitter materials
- Bio Chromatography, horizontal circular images, minerals, sugars/ bitter materials
