Difference between revisions of "Circular chromatography"
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===Materials=== | ===Materials=== | ||
'''Chemicals''' | '''Chemicals''' | ||
| − | * 0.5% solution of silver nitrate | + | * 0.5% solution of silver nitrate (you can find in photography suppliers) |
| − | * 0.1 to 1% solution of sodium hydroxide | + | * 0.1 to 1% solution of [https://en.wikipedia.org/wiki/Sodium_hydroxide sodium hydroxide] (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn) |
'''Other materials''' | '''Other materials''' | ||
| − | * paper | + | * paper (Whatman, or any acid-free thick paper) |
* reservoir | * reservoir | ||
| − | * wick | + | * wick (medical cotton, and rolled it into a wick) |
* light for development - sunlight is fine unless controlled exposure is wanted | * light for development - sunlight is fine unless controlled exposure is wanted | ||
<br> | <br> | ||
Revision as of 09:14, 3 April 2017
Contents
Introduction
CHROMATO = colour; of or in colours
GRAPHY = "writing" or a "field of study"
Methods
This is the recommended protocol from the page of BIO-DYNAMIC ASSOCIATION OF INDIA
Materials
Chemicals
- 0.5% solution of silver nitrate (you can find in photography suppliers)
- 0.1 to 1% solution of sodium hydroxide (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)
Other materials
- paper (Whatman, or any acid-free thick paper)
- reservoir
- wick (medical cotton, and rolled it into a wick)
- light for development - sunlight is fine unless controlled exposure is wanted
Step by Step
- A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter.
- The wick is removed and the paper is dried.
- Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time.
- The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper.
- When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop.
Which method to use
- Bio Crystallisation, copper chloride crystallisation, proteins
- Capillary Dynamolysis, vertical filters, ‘rising pictures’, sugars/ bitter materials
- Bio Chromatography, horizontal circular images, minerals, sugars/ bitter materials
