Difference between revisions of "Circular chromatography"
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===Materials=== | ===Materials=== | ||
| + | '''Chemicals''' | ||
| + | * 0.5% solution of silver nitrate | ||
| + | * 0.1 to 1% solution of sodium hydroxide | ||
| + | '''Other materials''' | ||
| + | * paper | ||
| + | * reservoir | ||
| + | * wick | ||
| + | * light for development | ||
| + | |||
===Step by Step=== | ===Step by Step=== | ||
# A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. | # A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter. | ||
Revision as of 12:04, 27 March 2017
Contents
Introduction
Methods
This is the recommended protocol from the page of BIO-DYNAMIC ASSOCIATION OF INDIA
Materials
Chemicals
- 0.5% solution of silver nitrate
- 0.1 to 1% solution of sodium hydroxide
Other materials
- paper
- reservoir
- wick
- light for development
Step by Step
- A circular filter paper (Whatman #1) with a cylindrical paper wick sitting in a 0.5% solution of silver nitrate is allowed to absorb the solution, which spreads by capillary action, to a certain diameter.
- The wick is removed and the paper is dried.
- Meanwhile, the substance to be tested is mixed with a 0.1 to 1% solution of sodium hydroxide and let stand for a period of time.
- The prepared filter paper is then allowed to absorb this solution and the substance spreads over the paper.
- When it has spread to a certain distance, the wick is removed and the paper dried. The paper is then exposed to indirect sunlight to let the image develop.
Which method to use
- Bio Crystallisation, copper chloride crystallisation, proteins
- Capillary Dynamolysis, vertical filters, ‘rising pictures’, sugars/ bitter materials
- Bio Chromatography, horizontal circular images, minerals, sugars/ bitter materials
