Circular chromatography - Revision history

Sachiko: /* Introduction */

2017-04-13T11:54:07Z

‎Introduction

2017-04-13T08:42:55Z

‎Analysis of the Chromatogram

2017-04-13T08:13:41Z

‎Introduction

2017-04-13T08:08:59Z

‎Materials

2017-04-13T08:07:19Z

‎Introduction

2017-04-10T11:21:51Z

‎Sample application

2017-04-10T11:21:05Z

‎Sample application

2017-04-10T10:59:32Z

‎Sample application

2017-04-10T10:25:51Z

‎Sample application

2017-04-10T10:21:19Z

‎Sample application

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 In this case, we took some soil and mud samples we had collected in different water bodies locations, and tried to analyse the humic content of the samples.<br>
 This method can be applied to any other liquid mixtures.<br>
-For example, [https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0ahUKEwizscXL-qDTAhXEpo8KHdVgC-8QFggkMAA&url=http%3A%2F%2Fjournal.iisc.ernet.in%2Findex.php%2Fiisc%2Farticle%2Fdownload%2F2786%2F4173&usg=AFQjCNE4N72bxFl2hwdMwSm47mFqaQ9xZw&sig2=ikNpi2l8nfCn-YukCpOseA&cad=rja this 1953 paper] uses butanol-based solvents to separate different sugars, and visualizes the different sugars. <br><br>
+For example, [https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0ahUKEwizscXL-qDTAhXEpo8KHdVgC-8QFggkMAA&url=http%3A%2F%2Fjournal.iisc.ernet.in%2Findex.php%2Fiisc%2Farticle%2Fdownload%2F2786%2F4173&usg=AFQjCNE4N72bxFl2hwdMwSm47mFqaQ9xZw&sig2=ikNpi2l8nfCn-YukCpOseA&cad=rja this 1953 paper] uses butanol-based solvents to separate different sugars, and visualizes the different sugars. <br>
 To learn more, here is the Khan Academy's explanation of the [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Principles of Chromatography].
 <br><br>

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 See one explanation at the bottom of this [http://www.biodynamics.in/chrom.htm webpage].
 <br>
 ===Which method to use===

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 <br>
 In this case, we took some soil and mud samples we had collected in different water bodies locations, and tried to analyse the humic content of the samples.<br>
-This method can be applied to any other liquid mixtures.<br><br>
+This method can be applied to any other liquid mixtures.<br>
 To learn more, here is the Khan Academy's explanation of the [https://www.khanacademy.org/test-prep/mcat/chemical-processes/separations-purifications/a/principles-of-chromatography Principles of Chromatography].
 <br><br>

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 ===Materials===
 ----
-'''Chemicals'''
+'''Chemicals''' (!! again, this depends on what you want to separate, and what compounds you want to see)
-* 0.5% solution of silver nitrate (you can find in photography suppliers)
+* '''for visualization''' - 0.5% solution of silver nitrate (you can find in photography suppliers)
-* 0.1 to 1% solution of [https://en.wikipedia.org/wiki/Sodium_hydroxide sodium hydroxide] (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)
+* '''solvent, mobile phase''' - 0.1 to 1% solution of [https://en.wikipedia.org/wiki/Sodium_hydroxide sodium hydroxide] (NaOH, otherwise known as lye or caustic soda - be careful, because it can burn)
 '''Other materials'''
 * paper (Whatman, or any acid-free thick paper)

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 The separation happens as the liquid slowly wets the paper by [https://en.wikipedia.org/wiki/Capillary_action capillary action] - <br>
 Those compounds that stick to the paper (medium/ '''stationary / solid phase'''), will stay closer to the point of inflow.<br>
-Those compounds that prefer the liquid ('''mobile phase'''), it will travel further with the liquid.<br>
+Those compounds that prefer the liquid ('''solvent''', '''mobile phase'''), it will travel further with the liquid.<br>
 This process can just be called [https://en.wikipedia.org/wiki/Paper_chromatography paper chromatography]. Because we use paper as a support.Because we did this on a circular piece of paper, it is called circular chromatography. <br>

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 # Take 2mL the liquid part of the samples prepared above, and place in a cleaned container
-[[File:Wik and solution.JPG]]
+[[File:Wik and solution.JPG|640px]]
 # Let the sample travel til just before the silver nitrate circle
-[[File:Dipping sample.JPG]]
+[[File:Dipping sample.JPG|640px]]
 # Remove wick
-[[File:Dipped paper.JPG]]
+[[File:Dipped paper.JPG|640px]]
 # Let the filter dry

@@ Line 93: / Line 93: @@
 # Add a new wick on the prepared paper above
-[[File:IMG 8929-wix and filter paper.JPG|640*427]]
+[[File:IMG 8929-wix and filter paper.JPG|640px]]
 # Take 2mL the liquid part of the samples prepared above, and place in a cleaned container